The human malaria parasite Plasmodium falciparum is responsible for the majority of malaria-related deaths. Tools allowing
the study of the basic biology of P. falciparum throughout the life cycle are critical to the development of new strategies to
target the parasite within both human and mosquito hosts. We here present 3D7HT-GFP, a strain of P. falciparum
constitutively expressing the Green Fluorescent Protein (GFP) throughout the life cycle, which has retained its capacity to
complete sporogonic development. The GFP expressing cassette was inserted in the Pf47 locus. Using this transgenic strain,
parasite tracking and population dynamics studies in mosquito stages and exo-erythrocytic schizogony is greatly facilitated.
The development of 3D7HT-GFP will permit a deeper understanding of the biology of parasite-host vector interactions, and
facilitate the development of high-throughput malaria transmission assays and thus aid development of new intervention
strategies against both parasite and mosquito.