Vitamin D attenuates rhinovirus-induced expression of intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAFR) in respiratory epithelial cells
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Human rhinoviruses commonly cause upper respiratory infections, which may be complicated by secondary bacterial infection. Vitamin D replacement reduces risk of acute respiratory infections in vitamin D-deficient individuals, but the mechanisms by which such protection is mediated are incompletely understood. We therefore conducted experiments to characterise the influence of the major circulating metabolite 25-hydroxyvitamin D (25[OH]D) and the active metabolite 1,25-dihydroxyvitamin D (1,25[OH]2D) on responses of a respiratory epithelial cell line (A549 cells) to infection with a major group human rhinovirus (RV-16). Pre-treatment of A549 respiratory epithelial cells with a physiological concentration (10-7M) of 25(OH)D induced transient resistance to infection with RV-16 and attenuated RV-16-induced expression of the genes encoding intercellular adhesion molecule 1 (ICAM-1, a cell surface glycoprotein that acts as the cellular receptor for major group rhinoviruses) and platelet-activating factor receptor (PAFR, a G-protein coupled receptor implicated in adhesion of Streptococcus pneumoniae to respiratory epithelial cells). These effects were associated with enhanced expression of the genes encoding the NF-κB inhibitor IκBα and the antimicrobial peptide cathelicidin LL-37. Our findings suggest possible mechanisms by which vitamin D may enhance resistance to rhinovirus infection and reduce risk of secondary bacterial infection in vitamin D-deficient individuals.
Date Issued
2019-03-01
Date Acceptance
2018-11-23
Citation
Journal of Steroid Biochemistry and Molecular Biology, 2019, 187, pp.152-159
ISSN
0960-0760
Publisher
Elsevier
Start Page
152
End Page
159
Journal / Book Title
Journal of Steroid Biochemistry and Molecular Biology
Volume
187
Copyright Statement
Crown Copyright © 2018 Published by Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/30476590
PII: S0960-0760(18)30543-0
Subjects
1,25-dihydroxyvitamin D
25-hydroxyvitamin D
CAMP
Human rhinovirus
ICAM1
IKBA
PTAFR
Respiratory epithelial cells
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2018-11-23