Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
We report the characterisation of gated optical image intensifiers for fluorescence lifetime imaging
(FLIM), evaluating the performance of several different prototypes that culminate in a new design that
provides improved spatial resolution conferred b
y the addition of a magnetic field to reduce the lateral
spread of photoelectrons on their path between the photocathode and microchannel plate, and higher
signal to noise ratio conferred by longer time gates. We also present a methodology to compare these
systems and their capabilities, including the quantitative readouts of F
ö
rster resonant energy transfer
(FRET).
(FLIM), evaluating the performance of several different prototypes that culminate in a new design that
provides improved spatial resolution conferred b
y the addition of a magnetic field to reduce the lateral
spread of photoelectrons on their path between the photocathode and microchannel plate, and higher
signal to noise ratio conferred by longer time gates. We also present a methodology to compare these
systems and their capabilities, including the quantitative readouts of F
ö
rster resonant energy transfer
(FRET).
Date Issued
2017-01-26
Date Acceptance
2016-12-26
Citation
Review of Scientific Instruments, 2017, 88
ISSN
1089-7623
Publisher
AIP Publishing
Journal / Book Title
Review of Scientific Instruments
Volume
88
Copyright Statement
© 2017 Author(s). All article content, except
where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license
(http://creativecommons.org/licenses/by/4.0/).
where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license
(http://creativecommons.org/licenses/by/4.0/).
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council
Grant Number
EP/I02770X/1
BB/M006786/1
BB/M006786/1
Subjects
Science & Technology
Technology
Physical Sciences
Instruments & Instrumentation
Physics, Applied
Physics
PLATE READER
TIME-DOMAIN
MICROSCOPY
SPEED
FLIM
FRET
EXCITATION
RESOLUTION
SYSTEM
LIGHT
Applied Physics
09 Engineering
02 Physical Sciences
03 Chemical Sciences
Publication Status
Published
Article Number
013707