Studies of proteasome inhibition and apoptosis induction in triple‐negative breast cancer cells by novel amino acid–polypyridine–copper complex
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
An innovative ternary copper(II) complex, [Cu(Cl‐PIP)(Tyr)Cl]n, has been synthesized and characterized using infrared spectroscopy, elemental analysis and single‐crystal X‐ray diffraction analysis. X‐ray crystallography indicates that the Cu atom is five‐coordinated in a square‐pyramidal configuration. The unit forms a one‐dimensional chain along the crystallographic c‐axis. The complex was screened for cytotoxicity against a panel of eight human cancer cell lines, namely MDA‐MB‐231, CAL‐51, K562, HeLa, SGC‐7901, A549, MCF‐7 and SMMC‐7721. The best anticancer activity was obtained with triple‐negative breast cancer CAL‐51 and MDA‐MB‐231 cell lines, with IC50 values in the range 0.035–0.10 μM, and this was better than using carboplatin. The complex inhibits proteasomal chymotrypsin‐like activity, and docking studies reveal its interaction with 20S proteasome. In addition, the complex causes accumulation of ubiquitinated proteins, induces apoptosis and inhibits cell proliferation, indicating its great potential as a novel therapy for triple‐negative breast cancer.
Date Issued
2019-10-01
Date Acceptance
2019-06-25
Citation
Applied Organometallic Chemistry, 2019, 33 (10)
ISSN
0268-2605
Publisher
Wiley
Journal / Book Title
Applied Organometallic Chemistry
Volume
33
Issue
10
Copyright Statement
© 2019 John Wiley & Sons, Ltd. This is the accepted version of the following article: Li, D, Wang, L, Yagüe, E, et al. Studies of proteasome inhibition and apoptosis induction in triple‐negative breast cancer cells by novel amino acid–polypyridine–copper complex. Appl Organometal Chem. 2019;e5120, which has been published in final form at https://doi.org/10.1002/aoc.5120
Sponsor
Breast Cancer Care & Breast Cancer Now
Identifier
https://onlinelibrary.wiley.com/doi/full/10.1002/aoc.5120
Grant Number
2014NovPhD326
Subjects
Inorganic & Nuclear Chemistry
0302 Inorganic Chemistry
0305 Organic Chemistry
0399 Other Chemical Sciences
Publication Status
Published
Article Number
ARTN e5120
Date Publish Online
2019-07-22