Fast, easy and early (larval) identification of transparent mutant zebrafish using standard fluorescence microscopy [version 1; peer review: 2 approved]
File(s)
Author(s)
Wenz, Ralf
Conibear, Emily
Bugeon, Laurence
Dallman, Maggie
Type
Journal Article
Abstract
The availability of transparent zebrafish mutants (either TraNac: tra b6/b6; nac w2/w2 or casper: roy a9/a9; nac w2/w2 ) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent casper ( roy a9/a9;nac w2/w2) and silver nacre ( nac w2/w2) mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide.
Date Issued
2020-08-10
Date Acceptance
2020-07-09
Citation
F1000Research, 2020, 9
ISSN
2046-1402
Publisher
F1000 Research Ltd
Journal / Book Title
F1000Research
Volume
9
Copyright Statement
© 2020 Wenz R et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32934809
Subjects
Zebrafish
casper
iridophore
nac
screening
tra
trab6/b6nacw2/w2
translucent
transparent
Publication Status
Published online
Coverage Spatial
England
Article Number
ARTN 963