Spot and cumulative urine samples are suitable replacements for 24-hour urine collections for objective measures of dietary exposure in adults using metabolite biomarkers
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
BACKGROUND: Measurement of multiple food intake exposure biomarkers in urine may offer an objective method for monitoring diet. The potential of spot and cumulative urine samples that have reduced burden on participants as replacements for 24-h urine collections has not been evaluated. OBJECTIVE: The aim of this study was to determine the utility of spot and cumulative urine samples for classifying the metabolic profiles of people according to dietary intake when compared with 24-h urine collections in a controlled dietary intervention study. METHODS: Nineteen healthy individuals (10 male, 9 female, aged 21-65 y, BMI 20-35 kg/m2) each consumed 4 distinctly different diets, each for 1 wk. Spot urine samples were collected ∼2 h post meals on 3 intervention days/wk. Cumulative urine samples were collected daily over 3 separate temporal periods. A 24-h urine collection was created by combining the 3 cumulative urine samples. Urine samples were analyzed with metabolite fingerprinting by both high-resolution flow infusion electrospray mass spectrometry (FIE-HRMS) and proton nuclear magnetic resonance spectroscopy (1H-NMR). Concentrations of dietary intake biomarkers were measured with liquid chromatography triple quadrupole mass spectrometry and by integration of 1H-NMR data. RESULTS: Cross-validation modeling with 1H-NMR and FIE-HRMS data demonstrated the power of spot and cumulative urine samples in predicting dietary patterns in 24-h urine collections. Particularly, there was no significant loss of information when post-dinner (PD) spot or overnight cumulative samples were substituted for 24-h urine collections (classification accuracies of 0.891 and 0.938, respectively). Quantitative analysis of urine samples also demonstrated the relation between PD spot samples and 24-h urines for dietary exposure biomarkers. CONCLUSIONS: We conclude that PD spot urine samples are suitable replacements for 24-h urine collections. Alternatively, cumulative samples collected overnight predict similarly to 24-h urine samples and have a lower collection burden for participants.
Date Issued
2019-10-01
Date Acceptance
2019-05-24
Citation
Journal of Nutrition, 2019, 149 (10), pp.1692-1700
ISSN
0022-3166
Publisher
Oxford University Press
Start Page
1692
End Page
1700
Journal / Book Title
Journal of Nutrition
Volume
149
Issue
10
Copyright Statement
© 2019 Oxford University Press. This is a pre-copy-editing, author-produced version of an article accepted for publication in Journal of Nutrition following peer review. The definitive publisher-authenticated version Thomas Wilson, Isabel Garcia-Perez, Joram M Posma, Amanda J Lloyd, Edward S Chambers, Kathleen Tailliart, Hassan Zubair, Manfred Beckmann, John C Mathers, Elaine Holmes, Gary Frost, John Draper, Spot and Cumulative Urine Samples Are Suitable Replacements for 24-Hour Urine Collections for Objective Measures of Dietary Exposure in Adults Using Metabolite Biomarkers, The Journal of Nutrition, , nxz138, https://doi.org/10.1093/jn/nxz138 is available online at: https://doi.org/10.1093/jn/nxz138.
Sponsor
National Institute for Health Research
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31240300
PII: 5523192
Grant Number
NIHR-PDF-2012-05-456
Subjects
24-h urine
dietary intake
high-resolution mass spectrometry
metabolomics
nuclear magnetic resonance spectroscopy
spot urine
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2019-06-26