Surface presentation of functional peptides in solution determines cell internalization efficiency of TAT conjugated nanoparticles
File(s)TodorovaN-Nanoletters-2014-accepted-version.docx (766.77 KB)
Accepted version
Author(s)
Type
Journal Article
Abstract
Functionalizing nanoparticles with cell-penetrating peptides is a popular choice for cellular delivery. We investigated the effects of TAT peptide concentration and arrangement in solution on functionalized nanoparticles’ efficacy for membrane permeation. We found that cell internalization correlates with the positive charge distribution achieved prior to nanoparticle encountering interactions with membrane. We identified a combination of solution based properties required to maximize the internalization efficacy of TAT-functionalized nanoparticles.
Date Issued
2014-08-26
Date Acceptance
2014-08-01
Citation
Nano Letters, 2014, 14 (9), pp.5229-5237
ISSN
1530-6984
Publisher
American Chemical Society
Start Page
5229
End Page
5237
Journal / Book Title
Nano Letters
Volume
14
Issue
9
Copyright Statement
© 2014 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/nl5021848
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
Cell internalization
functionalized nanoparticles
gold
cell-penetrating peptides
molecular dynamics
MODIFIED GOLD NANOPARTICLES
MONOLAYER-PROTECTED NANOPARTICLES
PENETRATING PEPTIDES
INTRACELLULAR DELIVERY
LIPID-BILAYERS
SIZE
LIPOSOMES
PROTEINS
DOMAINS
BIOLOGY
Publication Status
Published