Protocol for a semi-quantitative approach to identify protein S-palmitoylation in cultured cells by acyl biotin exchange assay
File(s)
Author(s)
Leishman, Stuart
Aljadeed, Najd M
Anand, Paras K
Type
Journal Article
Abstract
Main text
(STAR Protocols 5, 103054; June 21, 2024)
During manuscript preparation, two concentration volumes were inadvertently misreported in the “materials and equipment” and “step-by-step method details” sections of this protocol.
• Under “materials and equipment,” (1) the stock concentration for hydroxylamine reads “32 mM” while it should read “32 M,” and (2) the final concentration for biotin-HPDP reads “1.0 M” while it should read “1.0 mM.”
• In step 32 of “step-by-step method details,” the “20 mM Biotin-HPDP” that is mentioned should instead read “Biotin-HPDP to 1 mM final concentration” due to the above changes.
The authors apologize for the errors and regret any confusion caused.
(STAR Protocols 5, 103054; June 21, 2024)
During manuscript preparation, two concentration volumes were inadvertently misreported in the “materials and equipment” and “step-by-step method details” sections of this protocol.
• Under “materials and equipment,” (1) the stock concentration for hydroxylamine reads “32 mM” while it should read “32 M,” and (2) the final concentration for biotin-HPDP reads “1.0 M” while it should read “1.0 mM.”
• In step 32 of “step-by-step method details,” the “20 mM Biotin-HPDP” that is mentioned should instead read “Biotin-HPDP to 1 mM final concentration” due to the above changes.
The authors apologize for the errors and regret any confusion caused.
Date Issued
2026-06-07
Date Acceptance
2024-05-01
Citation
STAR Protocols, 2026, 7 (2)
ISSN
2666-1667
Publisher
Elsevier BV
Journal / Book Title
STAR Protocols
Volume
7
Issue
2
Copyright Statement
© 2026 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Publication Status
Published
Article Number
104638
Date Publish Online
2026-06-10