The Irish Potato Famine pathogen phytophthora infestans translocates the CRN8 kinase into host plant cells
Author(s)
Type
Journal Article
Abstract
Phytophthora infestans is the causal agent of late blight on potato and tomato. It is now well established that oomycetes, such as P. infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. The Crinkler (CRN) effector family containing the LFLAK translocation motif represents a new class of cytoplasmic effectors from oomycetes. The N-terminal domain, which includes this motif, is dispensable for cell death induction in planta, indicating the presence of modular domains that are involved in distinct processes. The C-terminal/effector domain of CRN8 localizes to the host nucleus and nuclear localization is required for triggering cell death. The CRN8 effector domain has similarity to plant Serine/Threonine RD kinases. This intriguing finding raises the possibility that CRN8 mimics a specific class of plant enzymes, a feature that has been observed for several other pathogen effectors. Using biochemical assays, we confirmed that the C-terminal domain of CRN8 has kinase activity, and have identified by mass spectrometry five serines shown to be auto-phosphorylated. To the best of our knowledge, CRN8 is the first reported secreted active kinase from a microbial plant pathogen.
Date Issued
2012-08-23
Date Acceptance
2012-07-10
Citation
PLoS Pathogens, 2012, 8 (8), pp.1-12
ISSN
1553-7366
Publisher
Public Library of Science (PLoS)
Start Page
1
End Page
12
Journal / Book Title
PLoS Pathogens
Volume
8
Issue
8
Copyright Statement
© van Damme et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000308558000049&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
Parasitology
Virology
TOXOPLASMA-GONDII
PROTEIN-KINASE
III EFFECTORS
TYROSINE-PHOSPHATASE
OOMYCETE EFFECTORS
MOLECULAR-CLONING
VIRULENCE FACTOR
RHOPTRY PROTEIN
INNATE IMMUNITY
RXLR EFFECTORS
Publication Status
Published
Article Number
ARTN e1002875
Date Publish Online
2012-08-23