Exosomal miRNAs and association with pulmonary tuberculosis
File(s)
Author(s)
Mortaz, Esmaeil
Alipoor, Shamila D
Folkerts, Gert
Garssen, Johan
Adcock, Ian
Type
Conference Paper
Abstract
Introduction: Tuberculosis (TB) remains a major threat to human health. Due to the limited accuracy of the current TB diagnostic tests, it is critical to determine novel biomarkers for this disease. Circulating exosomes have been used as diagnostic biomarkers in various diseases.
Objective of the Study: In this study, we examined the expression of miRNAs as biomarker candidates for the diagnosis of TB infection.
Methods: Serum exosomes were isolated using the total exosome isolation (TEI) reagent (Invitrogen, Thermo Fisher Scientific Corporation, USA). Serum-derived exosomes were isolated from TB patients (25 new case of active TB) and matched control subjects (25 persons). The expression of miR-484, miR-425, and miR-96 was examined by RT-PCR.
Results: The expression of miR-484, miR-425, and miR-96 were significantly increased in serum of TB patients which correlated with the TB infection level. After normalization to U6, a statistically significant upregulation of miR-484 (13 55 ± 3 44 − fold increase, p ≤ 0 01), miR-425 (6 84 ± 1 7 − fold increase, p ≤ 0 01), and miR-96 (2 37 ± 0 53 − fold increase, p ≤ 0 05) was demonstrated in TB patients in comparison to healthy controls. A receiver operating characteristic (ROC) curve analysis showed the diagnostic potency of each individual serum exosomal miRNA with an area under the curve AUC =072 for miR-484 (p<005), 0.66 for miR-425 (p<005), and 0.62 for miR-96 (p<005).
Conclusion: These results demonstrate that exosomal miRNAs have diagnostic potential in active tuberculosis. The diagnostic power may be improved when combined with conventional diagnostic markers.
Objective of the Study: In this study, we examined the expression of miRNAs as biomarker candidates for the diagnosis of TB infection.
Methods: Serum exosomes were isolated using the total exosome isolation (TEI) reagent (Invitrogen, Thermo Fisher Scientific Corporation, USA). Serum-derived exosomes were isolated from TB patients (25 new case of active TB) and matched control subjects (25 persons). The expression of miR-484, miR-425, and miR-96 was examined by RT-PCR.
Results: The expression of miR-484, miR-425, and miR-96 were significantly increased in serum of TB patients which correlated with the TB infection level. After normalization to U6, a statistically significant upregulation of miR-484 (13 55 ± 3 44 − fold increase, p ≤ 0 01), miR-425 (6 84 ± 1 7 − fold increase, p ≤ 0 01), and miR-96 (2 37 ± 0 53 − fold increase, p ≤ 0 05) was demonstrated in TB patients in comparison to healthy controls. A receiver operating characteristic (ROC) curve analysis showed the diagnostic potency of each individual serum exosomal miRNA with an area under the curve AUC =072 for miR-484 (p<005), 0.66 for miR-425 (p<005), and 0.62 for miR-96 (p<005).
Conclusion: These results demonstrate that exosomal miRNAs have diagnostic potential in active tuberculosis. The diagnostic power may be improved when combined with conventional diagnostic markers.
Date Issued
2019-09-28
Date Acceptance
2019-09-01
Citation
European Respiratory Journal, 2019, 54, pp.1-2
ISSN
0903-1936
Publisher
European Respiratory Society
Start Page
1
End Page
2
Journal / Book Title
European Respiratory Journal
Volume
54
Copyright Statement
© the authors 2019. This is an author-submitted, peer-reviewed version of a manuscript that has been accepted for publication in the European Respiratory Journal, prior to copy-editing, formatting and typesetting. This version of the manuscript may not be duplicated or reproduced without prior permission from the copyright owner, the European Respiratory Society. The publisher is not responsible or liable for any errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final, copy-edited, published article, which is the version of record, is available without a subscription 18 months after the date of issue publication.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000507372407154&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Source
European-Respiratory-Society (ERS) International Congress
Subjects
Science & Technology
Life Sciences & Biomedicine
Respiratory System
Biomarkers
Immunology
Diagnosis
Publication Status
Published
Start Date
2019-09-28
Finish Date
2019-10-02
Coverage Spatial
Madrid, SPAIN
Date Publish Online
2019-11-21