This article introduces various aspects of technology business incubators (TBIs), emphasizing their increasing role in the spatial context where they are used as platforms and drivers of regional entrepreneurial ecosystems. It outlines the key themes of the emerging role of TBIs in sustainable regional development: TBI biodiversity of ecosystems; accelerating startups in the entrepreneurial university; TBI mechanisms challenged by green technology to sustain regional development; and TBIs’ connecting role between entrepreneurship education, experiential knowledge, and regional development. It proposes an agenda for future research on the role of TBIs as bridging mechanisms and drivers of entrepreneurship and regional development.