Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis
Author(s)
Type
Journal Article
Abstract
Published by Oxford University Press on behalf of the Society for Experimental Biology. Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (<10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (>100 samples d -1) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.
Date Issued
2018-04-27
Date Acceptance
2018-02-02
Citation
Journal of Experimental Botany, 2018, 69 (10), pp.2569-2579
ISSN
0022-0957
Publisher
Oxford University Press
Start Page
2569
End Page
2579
Journal / Book Title
Journal of Experimental Botany
Volume
69
Issue
10
Copyright Statement
© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Identifier
https://academic.oup.com/jxb/article/69/10/2569/4919650
Subjects
Arabidopsis
Chromatography, Liquid
High-Throughput Nucleotide Sequencing
Indoleacetic Acids
Multivariate Analysis
Mutation
Plant Proteins
Solid Phase Extraction
Tandem Mass Spectrometry
Arabidopsis
Indoleacetic Acids
Plant Proteins
Chromatography, Liquid
Multivariate Analysis
Mutation
Tandem Mass Spectrometry
Solid Phase Extraction
High-Throughput Nucleotide Sequencing
0607 Plant Biology
0703 Crop and Pasture Production
0604 Genetics
Plant Biology & Botany
Publication Status
Published
Date Publish Online
2018-03-03