In this article, we explore the opportunities and challenges of landscape approaches through the lens of responsible research and innovation (RRI). We use the case of transport biofuels to reflect on the capacity of landscape approaches to support the governance of emerging technologies. The case study, developed in the region of Sardinia, Italy, consists of a landscape design process for the implementation of biofuel technologies that rely on the use of non-food dedicated crops and agricultural residues. By using non-food feedstocks, the biofuel project aims to avoid competition with food production and achieve sustainability goals. Through the discussion of key dimensions of RRI in relation to this case, the article puts forward a set of critical aspects of landscape design processes that require further attention from theorists and practitioners in the field of landscape-based planning. These include the power imbalance that exists between the diverse actors involved in project activities, a need for improving the flexibility of the configuration of socio-technical systems, revising assumptions on ‘valid’ knowledge, and improving the deliberative component of planning processes.