Cell type of origin dictates the route to pluripotency
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources.
Date Issued
2017-12-05
Date Acceptance
2017-11-08
Citation
Cell Reports, 2017, 21 (10), pp.2649-2660
ISSN
2211-1247
Publisher
Elsevier
Start Page
2649
End Page
2660
Journal / Book Title
Cell Reports
Volume
21
Issue
10
Copyright Statement
© 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000417146000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Cell Biology
HEMATOPOIETIC STEM-CELLS
MASS CYTOMETRY
MOLECULAR ROADMAP
SOMATIC-CELLS
IPS CELLS
EXPRESSION
MARKERS
MOUSE
Publication Status
Published