The proteome of extracellular vesicles produced by the human gut bacteria bacteroides thetaiotaomicron in vivo Is influenced by environmental and host-derived factors
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Bacterial extracellular vesicles (BEVs) released from both Gram-negative and Gram-positive bacteria provide an effective means of communication and trafficking of cell signaling molecules. In the gastrointestinal tract (GIT) BEVs produced by members of the intestinal microbiota can impact host health by mediating microbe-host cell interactions. A major unresolved question, however, is what factors influence the composition of BEV proteins and whether the host influences protein packaging into BEVs and secretion into the GIT. To address this, we have analyzed the proteome of BEVs produced by the major human gut symbiont Bacteroides thetaiotaomicron both in vitro and in vivo in the murine GIT in order to identify proteins specifically enriched in BEVs produced in vivo. We identified 113 proteins enriched in BEVs produced in vivo, the majority (62/113) of which accumulated in BEVs in the absence of any changes in their expression by the parental cells. Among these selectively enriched proteins, we identified dipeptidyl peptidases and an asparaginase and confirmed their increased activity in BEVs produced in vivo. We also showed that intact BEVs are capable of degrading bile acids via a bile salt hydrolase. Collectively these findings provide additional evidence for the dynamic interplay of host-microbe interactions in the GIT and the existence of an active mechanism to drive and enrich a selected group of proteins for secretion into BEVs in the GIT.
Date Issued
2022-08-02
Date Acceptance
2022-07-11
Citation
Applied and Environmental Microbiology, 2022, 88 (16)
ISSN
0099-2240
Publisher
American Society for Microbiology
Journal / Book Title
Applied and Environmental Microbiology
Volume
88
Issue
16
Copyright Statement
© 2022 Stentz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000835643000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Microbiology
bacterial extracellular vesicles
proteome
intestine
microbiota
Bacteroides thetaiotaomicron
OUTER-MEMBRANE VESICLES
POLYSACCHARIDE UTILIZATION
IV
COMMUNICATION
ASPARAGINASE
PROTEINS
Publication Status
Published
Article Number
ARTN e00533-22