A rapid colorimetric method to visualize protein interactions
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
As key molecules in most biological pathways, proteins physically contact one or more biomolecules in a highly specific manner. Several driving forces (i.e., electrostatic and hydrophobic) facilitate such interactions and a variety of methods have been developed to monitor these processes both in vivo and in vitro. In this work, a new method is reported for the detection of protein interactions by visualizing a color change of a cyanine compound, a supramolecule complex of 3,3-di-(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC). Nuclear magnetic resonance (NMR) studies suggest that the hydrophobic nature of the protein surfaces drives MTC into different types of aggregates with distinct colors. When proteins interact with other biomolecules, the hydrophobic surface of the complex differs, resulting in a shift in the form of MTC aggregation, which results in a color change. As a result, this in vitro method has the potential to become a rapid tool for the confirmation of protein-biomolecule interactions, without the requirements for sophisticated instrumentation or approaches.
Date Issued
2018-05-07
Date Acceptance
2018-01-26
Citation
Chemistry - A European Journal, 2018, 24 (26), pp.6727-6731
ISSN
0947-6539
Publisher
Wiley
Start Page
6727
End Page
6731
Journal / Book Title
Chemistry - A European Journal
Volume
24
Issue
26
Copyright Statement
© 2018 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the pre-peer reviewed version of the following article, which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201800401
Sponsor
Wellcome Trust
Wellcome Trust
Medical Research Council (MRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/29505156
Grant Number
100958/Z/13/Z
100280/Z/12/Z
MR/P028225/1
Subjects
NMR spectroscopy
colorimetry
protein-protein interactions
supramolecular chemistry
Publication Status
Published
Coverage Spatial
Germany
Date Publish Online
2018-03-05