The structures of natively assembled clathrin-coated vesicles
File(s)
Author(s)
Type
Journal Article
Abstract
Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.
Date Issued
2020-07-22
Date Acceptance
2020-06-05
Citation
Science Advances, 2020, 6 (30)
ISSN
2375-2548
Publisher
American Association for the Advancement of Science
Journal / Book Title
Science Advances
Volume
6
Issue
30
Copyright Statement
© 2020 The Authors, some rights reserved; exclusive licensee
American Association for the Advancement of Science. No claim to
original U.S. Government Works. Distributed under a Creative
Commons Attribution License 4.0 (CC BY). This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
American Association for the Advancement of Science. No claim to
original U.S. Government Works. Distributed under a Creative
Commons Attribution License 4.0 (CC BY). This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32743076
PII: aba8397
Publication Status
Published
Coverage Spatial
United States
Article Number
ARTN eaba8397