Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Streptococcus suis is a bacterium commonly carried in the respiratory tract that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities efficacious vaccines are not readily available. The selection of S. suis protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent S. suis While subunit vaccination with the S. suis proteins induced IgG antibody titers to each individual protein, a cellular immune response to the pool of proteins, and provided substantial protection from challenge with virulent S. suis, the immune response elicited and degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different S. suis serotypes indicating a potential for cross-protection.
Date Issued
2018-01-03
Date Acceptance
2018-01-03
Citation
Infection and Immunity, 2018, 86 (3)
ISSN
0019-9567
Publisher
American Society for Microbiology
Journal / Book Title
Infection and Immunity
Volume
86
Issue
3
Copyright Statement
© 2018 American Society for Microbiology.
Identifier
PII: IAI.00559-17
Subjects
Science & Technology
Life Sciences & Biomedicine
Immunology
Infectious Diseases
Streptococcus suis
adjuvant
functional genomics
subunit
vaccine
GROUP-A STREPTOCOCCUS
EXTRACELLULAR FACTOR EF
CAPSULAR TYPES
DISEASE
GENES
PATHOGENESIS
PNEUMONIAE
POLYETHYLENEIMINE
POLYSACCHARIDE
COLONIZATION
BRADP1T Consortium
06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
Microbiology
Publication Status
Published
Article Number
e00559-17