Yeast expressed ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Recent advances in glycobiology have revealed the essential role of lectins in deciphering the glycocodes at the cell surface to generate important biological signaling responses. ArtinM, a d-mannose-binding lectin isolated from the seeds of jackfruit (Artocarpus heterophyllus), is composed of 16 kDa subunits that are associated to form a homotetramer. Native ArtinM (n-ArtinM) exerts immunomodulatory and regenerative effects, but the potential pharmaceutical applicability of the lectin is highly limited by the fact that its production is expensive, laborious, and impossible to be scaled up. This led us to characterize a recombinant form of the lectin obtained by expression in Saccharomyces cerevisiae (y-ArtinM). In the present study, we demonstrated that y-ArtinM is similar to n-ArtinM in subunit arrangement, oligomerization and carbohydrate binding specificity. We showed that y-ArtinM can exert n-ArtinM biological activities such as erythrocyte agglutination, stimulation of neutrophil migration and degranulation, mast cell degranulation, and induction of interleukin-12 and interleukin-10 production by macrophages. In summary, the expression of ArtinM in yeast resulted in successful production of an active, recombinant form of ArtinM that is potentially useful for pharmaceutical application.
Date Issued
2015-10-01
Date Acceptance
2015-09-29
Citation
International Journal of Biological Macromolecules, 2015, 82, pp.22-30
ISSN
0141-8130
Publisher
Elsevier
Start Page
22
End Page
30
Journal / Book Title
International Journal of Biological Macromolecules
Volume
82
Copyright Statement
© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND
license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Sponsor
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (E
Wellcome Trust
Wellcome Trust
Grant Number
N/a
EPSRC Grant EP/G037604/1
099197/Z/12/Z
108430/Z/15/Z
Subjects
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Applied
Polymer Science
Chemistry
ArtinM
Immunomodulatory lectins
Recombinant proteins
ARTOCARPUS-INTEGRIFOLIA SEEDS
MANNOSE-BINDING LECTIN
MAST-CELLS
KM PLUS
KM+
SPECIFICITY
ACTIVATION
INFECTION
BETA
RECEPTOR
Polymers
0601 Biochemistry And Cell Biology
Publication Status
Published