Image-guided Raman spectroscopy probe-tracking for tumor margin delineation
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Significance: Tumor detection and margin delineation are essential for successful tumor resection. However, postsurgical positive margin rates remain high for many cancers. Raman spectroscopy has shown promise as a highly accurate clinical spectroscopic diagnostic modality, but its margin delineation capabilities are severely limited by the need for pointwise application.
Aim: We aim to extend Raman spectroscopic diagnostics and develop a multimodal computer vision-based diagnostic system capable of both the detection and identification of suspicious lesions and the precise delineation of disease margins.
Approach: We first apply visual tracking of a Raman spectroscopic probe to achieve real-time tumor margin delineation. We then combine this system with protoporphyrin IX fluorescence imaging to achieve fluorescence-guided Raman spectroscopic margin delineation.
Results: Our system enables real-time Raman spectroscopic tumor margin delineation for both ex vivo human tumor biopsies and an in vivo tumor xenograft mouse model. We then further demonstrate that the addition of protoporphyrin IX fluorescence imaging enables fluorescence-guided Raman spectroscopic margin delineation in a tissue phantom model.
Conclusions: Our image-guided Raman spectroscopic probe-tracking system enables tumor margin delineation and is compatible with both white light and fluorescence image guidance, demonstrating the potential for our system to be developed toward clinical tumor resection surgeries.
Aim: We aim to extend Raman spectroscopic diagnostics and develop a multimodal computer vision-based diagnostic system capable of both the detection and identification of suspicious lesions and the precise delineation of disease margins.
Approach: We first apply visual tracking of a Raman spectroscopic probe to achieve real-time tumor margin delineation. We then combine this system with protoporphyrin IX fluorescence imaging to achieve fluorescence-guided Raman spectroscopic margin delineation.
Results: Our system enables real-time Raman spectroscopic tumor margin delineation for both ex vivo human tumor biopsies and an in vivo tumor xenograft mouse model. We then further demonstrate that the addition of protoporphyrin IX fluorescence imaging enables fluorescence-guided Raman spectroscopic margin delineation in a tissue phantom model.
Conclusions: Our image-guided Raman spectroscopic probe-tracking system enables tumor margin delineation and is compatible with both white light and fluorescence image guidance, demonstrating the potential for our system to be developed toward clinical tumor resection surgeries.
Date Issued
2021-03-13
Date Acceptance
2021-02-17
Citation
Journal of Biomedical Optics, 2021, 26 (3), pp.1-15
ISSN
1083-3668
Publisher
Society of Photo-optical Instrumentation Engineers
Start Page
1
End Page
15
Journal / Book Title
Journal of Biomedical Optics
Volume
26
Issue
3
Copyright Statement
© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.26.3.036002]
License URL
Sponsor
Commission of the European Communities
Commission of the European Communities
GlaxoSmithKline Services Unlimited
Wellcome Trust
Identifier
https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-26/issue-03/036002/Image-guided-Raman-spectroscopy-probe-tracking-for-tumor-margin-delineation/10.1117/1.JBO.26.3.036002.full?SSO=1
Grant Number
676137
701713
Mark Buswell
098411/Z/12/Z
Subjects
Raman spectroscopy
cancer
fluorescence guidance
margin delineation
probe tracking
Optics
0205 Optical Physics
0903 Biomedical Engineering
1113 Opthalmology and Optometry
Publication Status
Published
Article Number
036002
Date Publish Online
2021-03-13