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Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15–25 Å
File(s)
d4cc04524f.pdf (641.51 KB)
Published version
Author(s)
Girvan, Paul
Ying, Liming
Dodson, Charlotte A
Type
Journal Article
Abstract
Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.
Date Issued
2025-04-11
Date Acceptance
2025-02-27
Citation
Chemical Communications, 2025, 61 (28), pp.5353-5356
URI
URL
DOI
ISSN
1359-7345
Publisher
Royal Society of Chemistry (RSC)
Start Page
5353
End Page
5356
Journal / Book Title
Chemical Communications
Volume
61
Issue
28
Copyright Statement
© The Royal Society of Chemistry 2025. This article is licensed under aCreative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/)
License URL
Identifier
10.1039/d4cc04524f
Publication Status
Published
Date Publish Online
2025-03-04