Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation
Author(s)
Type
Journal Article
Abstract
Recent studies have shown that conjugation systems of Gram-negative bacteria are composed of distinct inner and outer membrane core complexes (IMCs and OMCCs, respectively). Here, we characterized the OMCC by focusing first on a cap domain that forms a channel across the outer membrane. Strikingly, the OMCC caps of the Escherichia coli pKM101 Tra and Agrobacterium tumefaciens VirB/VirD4 systems are completely dispensable for substrate transfer, but required for formation of conjugative pili. The pKM101 OMCC cap and extended pilus also are dispensable for activation of a Pseudomonas aeruginosa type VI secretion system (T6SS). Chimeric conjugation systems composed of the IMCpKM101 joined to OMCCs from the A. tumefaciens VirB/VirD4, E. coli R388 Trw, and Bordetella pertussis Ptl systems support conjugative DNA transfer in E. coli and trigger P. aeruginosa T6SS killing, but not pilus production. The A. tumefaciens VirB/VirD4 OMCC, solved by transmission electron microscopy, adopts a cage structure similar to the pKM101 OMCC. The findings establish that OMCCs are highly structurally and functionally conserved – but also intrinsically conformationally flexible – scaffolds for translocation channels. Furthermore, the OMCC cap and a pilus tip protein coregulate pilus extension but are not required for channel assembly or function.
Date Issued
2017-07-01
Date Acceptance
2017-04-27
Citation
Molecular Microbiology, 2017, 105 (2), pp.273-293
ISSN
0950-382X
Publisher
Wiley
Start Page
273
End Page
293
Journal / Book Title
Molecular Microbiology
Volume
105
Issue
2
Copyright Statement
© 2017 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000404871400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
AGROBACTERIUM VIRB10
Biochemistry & Molecular Biology
BRUCELLA-SUIS VIRB8
CONJUGAL TRANSFER SYSTEM
CORE COMPLEX
DNA TRANSFER
F-PILUS
GENETIC-ANALYSIS
HELICOBACTER-PYLORI CAGL
Life Sciences & Biomedicine
Microbiology
MUTATIONAL ANALYSIS
Science & Technology
T-DNA
Publication Status
Published
Date Publish Online
2017-04-25