Functional genomics in pancreatic β cells: recent advances in gene deletion and genome editing technologies for diabetes research.
File(s)
Published version
Author(s)
Hu, Ming
Cherkaoui, Ines
Misra, Shivani
Rutter, Guy A
Type
Journal Article
Abstract
The inheritance of variants that lead to coding changes in, or the mis-expression of, genes critical to pancreatic beta cell function can lead to alterations in insulin secretion and increase the risk of both type 1 and type 2 diabetes. Recently developed clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene editing tools provide a powerful means of understanding the impact of identified variants on cell function, growth, and survival and might ultimately provide a means, most likely after the transplantation of genetically "corrected" cells, of treating the disease. Here, we review some of the disease-associated genes and variants whose roles have been probed up to now. Next, we survey recent exciting developments in CRISPR/Cas9 technology and their possible exploitation for β cell functional genomics. Finally, we will provide a perspective as to how CRISPR/Cas9 technology may find clinical application in patients with diabetes.
Date Issued
2020-10
Date Acceptance
2020-09-17
Citation
Front Endocrinol (Lausanne), 2020, 11, pp.1-20
ISSN
1664-2392
Start Page
1
End Page
20
Journal / Book Title
Front Endocrinol (Lausanne)
Volume
11
Copyright Statement
© 2020 Hu, Cherkaoui, Misra and Rutter. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Sponsor
MRC Programme Grant
Medical Research Council (MRC)
Wellcome Trust
Wellcome Trust
Wellcome Trust
Wellcome Trust
Medical Research Council (MRC)
Medical Research Council (MRC)
Medical Research Council (MRC)
Medical Research Council (MRC)
INNOVATIVE MEDICINES INITIATIVE
Diabetes UK
Medical Research Council (MRC)
Medical Research Council (MRC)
Diabetes UK
Biotechnology and Biological Sciences Research Council (BBSRC)
Diabetes UK
The Royal Society
Medical Research Council (MRC)
Medical Research Council (MRC)
European Foundation for the Study of Diabetes
European Foundation for the Study of Diabetes
European Foundation for the Study of Diabetes
Diabetes UK
Sun Pharmaceutical Industries Limited
Diabetes UK
Diabetes UK
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33162936
Grant Number
MR/R022259/1
MR/R010676/1
212625/Z/18/Z
097816/Z/11/ZR
105603/Z/14/Z
098424/Z/12/ZR
MR/K001981/1
MR/N020472/1
MR/L02036X/1
MR/L02036X/1
115881
15 / 0005275
MR/M012646/1
R26199/CN001
15821
BB/J015873/1
12/0004601
WM100078
MR/K023667/1
16-0323
N/A
n/a
15/0005374
N/A
BDA number 13/0004672
13/0004672
Subjects
beta cell
genome editing
genome-wide association studies
maturity onset of diabetes of the young
mouse models
stem cells
Publication Status
Published online
Coverage Spatial
Switzerland
Date Publish Online
2020-10-08