Post mortem mapping of connectional anatomy for the validation of diffusion MRI
File(s)
Published version
Author(s)
Yendiki, Anastasia
Aggarwal, Manisha
Axer, Markus
Howard, Amy FD
van Walsum, Anne-Marie van Cappellen
Type
Journal Article
Abstract
Diffusion MRI (dMRI) is a unique tool for the study of brain circuitry, as it allows us to image both the macroscopic trajectories and the microstructural properties of axon bundles in vivo. The Human Connectome Project ushered in an era of impressive advances in dMRI acquisition and analysis. As a result of these efforts, the quality of dMRI data that could be acquired in vivo improved substantially, and large collections of such data became widely available. Despite this progress, the main limitation of dMRI remains: it does not image axons directly, but only provides indirect measurements based on the diffusion of water molecules. Thus, it must be validated by methods that allow direct visualization of axons but that can only be performed in post mortem brain tissue. In this review, we discuss methods for validating the various features of connectional anatomy that are extracted from dMRI, both at the macro-scale (trajectories of axon bundles), and at micro-scale (axonal orientations and other microstructural properties). We present a range of validation tools, including anatomic tracer studies, Klingler's dissection, myelin stains, label-free optical imaging techniques, and others. We provide an overview of the basic principles of each technique, its limitations, and what it has taught us so far about the accuracy of different dMRI acquisition and analysis approaches.
Date Issued
2022-08-01
Date Acceptance
2022-03-23
Citation
NeuroImage, 2022, 256
ISSN
1053-8119
Publisher
Elsevier
Journal / Book Title
NeuroImage
Volume
256
Copyright Statement
© 2022 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/35346838
PII: S1053-8119(22)00273-7
Subjects
AXON DIAMETER DISTRIBUTION
HISTOLOGICAL VALIDATION
HUMAN CONNECTOME PROJECT
HUMAN WHITE-MATTER
IN-VIVO
Life Sciences & Biomedicine
LONG-FIBER TRACTS
MAGNETIC-RESONANCE
Neuroimaging
Neurosciences
Neurosciences & Neurology
OPTICAL COHERENCE TOMOGRAPHY
POSTMORTEM HUMAN BRAINS
Radiology, Nuclear Medicine & Medical Imaging
Science & Technology
STRUCTURE TENSOR ANALYSIS
Publication Status
Published
Coverage Spatial
United States
Article Number
119146
Date Publish Online
2022-03-25