Magnetically retrievable platinum nanoreporters for efficient lateral flow immunoassay in complex bio-samples
Author(s)
Type
Journal Article
Abstract
Lateral flow immunoassays (LFIAs) are widely used for point-of-care diagnostics, but their development is challenged by the complexity and variability of patient samples. In particular, LFIAs often exhibit reduced sensitivity and specificity when used with patient samples, compared to their performance with analyte-spiked idealized matrices. Patient samples are inherently complex, with variations in physical and biochemical properties between patients. This complexity has consequences for the performance of LFIAs, and can result in non-specific binding on the test line, discoloration of the nitrocellulose membrane, and incomplete sample flow along the test strip. To address these challenges, a magnetically retrievable platinum nanoreporter (termed Pt@Fe3O4) is developed for LFIAs. Leveraging the magnetic properties of the Fe3O4 core, magnetic separation is utilized to enable the purification and concentration of target antigens from complex human matrices, including serum, saliva, and even stool samples. This also eliminates assay inconsistencies caused by inter-sample variability. Further, the suitability of Pt@Fe3O4 nanoreporters has been explored for use as detection probes in LFIAs. Signal enhancement is demonstrated by the utilization of the magnetic and enzyme-mimicking activity of the nanoreporter, resulting in a marked improvement in sensitivity, as evidenced by a 2- to 4-fold decrease in the visual limit of detection.
Date Issued
2026-01-08
Date Acceptance
2025-11-01
Citation
Small, 2026, 22 (2)
ISSN
1613-6810
Publisher
Wiley
Journal / Book Title
Small
Volume
22
Issue
2
Copyright Statement
© 2025 The Author(s). Small published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41311339
Subjects
Chemistry
Chemistry, Multidisciplinary
Chemistry, Physical
CORE
DEVICES
lateral flow immunoassay
magnetic separation
Materials Science
Materials Science, Multidisciplinary
matrix effect
NANOCOMPOSITES
NANOPARTICLES
Nanoscience & Nanotechnology
Physical Sciences
Physics
Physics, Applied
Physics, Condensed Matter
platinum nano-catalysts
Science & Technology
Science & Technology - Other Topics
SHELL
Technology
Publication Status
Published
Coverage Spatial
Germany
Article Number
e06622
Date Publish Online
2025-11-28