Positive Feedback Keeps Duration of Mitosis Temporally Insulated from Upstream Cell-Cycle Events
File(s)
Accepted version
Published version
Author(s)
Araujo, AR
Gelens, L
Sheriff, RSM
Santos, S
Type
Journal Article
Abstract
Cell division is characterized by a sequence of events by which a cell gives rise to two daughter cells. Quantitative measurements of cell-cycle dynamics in single cells showed that despite variability in G1-, S-, and G2 phases, duration of mitosis is short and remarkably constant. Surprisingly, there is no correlation between cell-cycle length and mitotic duration, suggesting that mitosis is temporally insulated from variability in earlier cell-cycle phases. By combining live cell imaging and computational modeling, we showed that positive feedback is the molecular mechanism underlying the temporal insulation of mitosis. Perturbing positive feedback gave rise to a sluggish, variable entry and progression through mitosis and uncoupled duration of mitosis from variability in cell cycle length. We show that positive feedback is important to keep mitosis short, constant, and temporally insulated and anticipate it might be a commonly used regulatory strategy to create modularity in other biological systems.
Date Issued
2016-10-20
Date Acceptance
2016-09-14
Citation
Molecular Cell, 2016, 64 (2), pp.362-375
ISSN
1097-4164
Publisher
Elsevier
Start Page
362
End Page
375
Journal / Book Title
Molecular Cell
Volume
64
Issue
2
Copyright Statement
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Subjects
cell division
mitosis
positive feedback
quantitative biology
temporal control
Developmental Biology
Biological Sciences
Medical And Health Sciences
Publication Status
Published