Spatial memory is a cognitive skill that allows the recall of information about the space, its layout, and items’ locations. We present a novel application built around 3D spatial audio technology to evaluate audio-spatial memory abilities. The sound sources have been spatially distributed employing the 3D Tune-In Toolkit, a virtual acoustic simulator. The participants are presented with sequences of sounds of increasing length emitted from virtual auditory sources around their heads. To identify stimuli positions and register the test responses, we designed a custom-made interface with buttons arranged according to sound locations. We took inspiration from the Corsi-Block test for the experimental procedure, a validated clinical approach for assessing visuo-spatial memory abilities. In two different experimental sessions, the participants were tested with the classical Corsi-Block and, blindfolded, with the proposed task, named Audio-Corsi for brevity. Our results show comparable performance across the two tests in terms of the estimated memory parameter precision. Furthermore, in the Audio-Corsi we observe a lower span compared to the Corsi-Block test. We discuss these results in the context of the theoretical relationship between the auditory and visual sensory modalities and potential applications of this system in multiple scientific and clinical contexts.