Evolutionary engineering improved D-Glucose/Xylose cofermentation of yarrowia lipolytica
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
The recombinant strain Y. lipolytica XYL+ can utilize xylose to produce microbial lipids efficiently. However, its xylose uptake is severely delayed in the presence of d-glucose. In this study, adaptive laboratory evolution (ALE) of strains in the medium containing xylose and d-glucose analogue 2-deoxyglucose (dG) is performed. After four stages of evolution over a total of 64 days, for the first time, we obtained an Y. lipolytica strain (yl-XYL+*04*10) that can cometabolize xylose and glucose, with diminished glucose repression. Xylose uptake kinetics showed that it could efficiently utilize xylose in the presence of 10 g/L of dG or d-glucose. Transcriptional profiling analysis revealed that the relative expression level of xylose-specific transporter genes (encoded by YALI0_C04730g and YALI0_D00363g) was significantly upregulated. In addition, missense mutations N373T and G270A in YALI0_E23287g (encoding a d-glucose transporter) and YALI0_E15488g (encoding a hexokinase) respectively, were found. It implied that these are important gene targets responsible for improved xylose utilization in evolved Y. lipolytica. Our work provides a new approach for breeding Y. lipolytica and paved the way for future pentose metabolic engineering.
Date Issued
2020-09-30
Date Acceptance
2020-06-01
Citation
Industrial and Engineering Chemistry Research, 2020, 59 (39), pp.17113-17123
ISSN
0888-5885
Publisher
American Chemical Society
Start Page
17113
End Page
17123
Journal / Book Title
Industrial and Engineering Chemistry Research
Volume
59
Issue
39
Copyright Statement
© 2020 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Ind. Eng. Chem. Res., after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.iecr.0c00896
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000577113500019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Technology
Engineering, Chemical
Engineering
XYLOSE UTILIZATION
LIPID PRODUCTION
TRANSPORTER
PLATFORM
COMPLEX
FUELS
Publication Status
Published
Date Publish Online
2020-06-01