In recent years, we have seen the arrival of Distributed Denial-of-Service (DDoS) open-source bot-based attack tools facilitating easy code enhancement, and so resulting in attack tools becoming more powerful. Developing new techniques for detecting and responding to the latest DDoS attacks often entails using attack traces to determine attack signatures and to test the techniques. However, obtaining actual attack traces is difficult, because the high-profile organizations that are typically attacked will not release monitored data as it may contain sensitive information. In this paper, we present a detailed study of the source code of the popular DDoS attack bots, Agobot, SDBot, RBot and Spybot to provide an in-depth understanding of the attacks in order to facilitate the design of more effective and efficient detection and mitigation techniques.