Experimental tools to reduce the burden of bacterial synthetic biology
File(s)
Accepted version
Author(s)
Grob, Alice
Di Blasi, Roberto
Ceroni, Francesca
Type
Journal Article
Abstract
Cellular burden limits the applications of bacterial synthetic biology. Experimental approaches for burden minimisation have recently become available. Tools to identify construct design with low footprint on the host include capacity monitors that quantify cellular capacity, high-throughput approaches and cell-free systems for construct prototyping. Orthogonal ribosomes and feedback controllers are instead useful to seek control of resource allocation and lower burden. Other approaches include genome reduction to increase the available resource pool and synthetic addiction to couple cell fitness and product accumulation. However, controlling the cellular response to exogenous expression is still a challenge, and more tools are needed in order to widen the applications of synthetic biology. Further effort that combines novel evolutionary data with burden-aware tools can set the foundation to increase the stability and robustness of future genetic systems.
Date Issued
2021-12-01
Date Acceptance
2021-10-08
Citation
Current Opinion in Systems Biology, 2021, 28
ISSN
2452-3100
Publisher
Elsevier
Journal / Book Title
Current Opinion in Systems Biology
Volume
28
Copyright Statement
© 2021 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://www.sciencedirect.com/science/article/abs/pii/S2452310021000883?via%3Dihub
Grant Number
BB/V00882X/1
Publication Status
Published
Article Number
ARTN 100393
Date Publish Online
2021-10-15