Bv8 mediates myeloid cell migration and enhances malignancy of colorectal cancer
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Colorectal cancer (CRC) is the third most predominant malignancy in the world. Although the importance of immune system in cancer development has been well established, the underlying mechanisms remain to be investigated further. Here we studied a novel protein prokineticin 2 (Prok2, also known as Bv8) as a key pro-tumoral factor in CRC progression in in vitro and ex vivo settings. Human colorectal tumor tissues, myeloid cell lines (U937 cells and HL60 cells) and colorectal cancer cell line (Caco-2 cells) were used for various studies. Myeloid cell infiltration (especially neutrophils) and Bv8 accumulation were detected in human colorectal tumor tissue with immunostaining. The chemotactic effects of Bv8 on myeloid cells were presented in the transwell assay and chemotaxis assy. Cultured CRC cells treated with myeloid cells or Bv8 produced reactive oxygen species (ROS) and vascular endothelial growth factor (VEGF). Furthermore, ROS and VEGF acted as pro-angiogenesis buffer in myeloid cell-infiltrated CRC microenvironment. Moreover, myeloid cells or Bv8 enhanced energy consumption of glycolysis ATP and mitochondria ATP of CRC cells. Interestingly, myeloid cells increased CRC cell viability, but CRC cells decreased the viability of myeloid cells. ERK signalling pathway in CRC cells was activated in the presence of Bv8 or co-cultured myeloid cells. In conclusion, our data indicated the vital roles of Bv8 in myeloid cell infiltration and CRC development, suggesting that Bv8 may be a potential therapeutic target for colorectal cancer-related immunotherapy.
Date Issued
2023-04-05
Date Acceptance
2023-03-27
Citation
Frontiers in Immunology, 2023, 14, pp.1-13
ISSN
1664-3224
Publisher
Frontiers Media S.A.
Start Page
1
End Page
13
Journal / Book Title
Frontiers in Immunology
Volume
14
Copyright Statement
Copyright © 2023 Li, Chang, Cui, Zhao, Hu, O’Dea, Tirlapur, Balboni, Zhang, Ying and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000970960800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
ACTIVATION
ANTIBODY
Bv8
colorectal cancer
EXPRESSION
GLUCOSE-UPTAKE
Immunology
INFLAMMATION
Life Sciences & Biomedicine
LOCALIZATION
malignancy
myeloid cells
NEUTROPHIL-DEPENDENT ANGIOGENESIS
OXIDATIVE STRESS
prokinectin 2
PROKINETICIN 2
RESISTANCE
Science & Technology
Publication Status
Published
Article Number
1158045
Date Publish Online
2023-04-05