A novel method for synthesis of carbon dots and their applications in reactive oxygen species (ROS) and glucose detections
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
A simple and novel method is proposed for preparation of water-soluble fluorescent carbon dots (C-dots), which have potential to be applied in detecting reactive oxygen species (ROS). The C-dots with high fluorescence quantum yield were created by hydrothermal methods with lactose as the carbon source and tris(hydroxylmethyl)aminomethane (Tris) as the surface passivation reagent. The C-dots have some unique characteristics such as excellent biocompatibility with a broad pH working range of 5–11 and high fluorescence, which makes them especially useful in the bio-detection field. The optical properties, surface groups, and element components of the prepared C-dots have been systematically studied by fluorescence spectroscopy. This facile approach is efficient and environmentally friendly and allows large-scale production of the C-dots without any further post-treatment. The C-dots have been adopted as probes for fluorescence turn-off detection owing to their high sensitivity to the hydroxyl radical. The detection limit can reach ∼0.1 μM under optimized conditions when using hydrogen peroxide as the source for generating ROS. Moreover, when paired with glucose oxidase, these C-dots can track glucose concentrations in samples. This adaptability suggests their potential in detecting various metabolites, paving the way for practical uses in disease detection.
Date Issued
2023
Date Acceptance
2023-09-11
Citation
RSC Advances: an international journal to further the chemical sciences, 2023, 13 (40), pp.28250-28261
ISSN
2046-2069
Publisher
The Royal Society of Chemistry
Start Page
28250
End Page
28261
Journal / Book Title
RSC Advances: an international journal to further the chemical sciences
Volume
13
Issue
40
Copyright Statement
© 2023 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
License URL
Identifier
http://dx.doi.org/10.1039/d3ra01795h
Publication Status
Published
Date Publish Online
2023-09-25