The cryoelectron microscopy structure of the Type 1 chaperone-usher pilus rod

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Author(s)

Hospenthal, Manuela K

Zyla, Dawid

Costa, Tiago RD

Redzej, Adam

Giese, Christoph

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Type

Journal Article

Abstract

Adhesive chaperone-usher pili are long, supramolecular protein fibers displayed on the surface of many bacterial pathogens. The type 1 and P pili of uropathogenic Escherichia coli (UPEC) play important roles during urinary tract colonization, mediating attachment to the bladder and kidney, respectively. The biomechanical properties of the helical pilus rods allow them to reversibly uncoil in response to flow-induced forces, allowing UPEC to retain a foothold in the unique and hostile environment of the urinary tract. Here we provide the 4.2-Å resolution cryo-EM structure of the type 1 pilus rod, which together with the previous P pilus rod structure rationalizes the remarkable “spring-like” properties of chaperone-usher pili. The cryo-EM structure of the type 1 pilus rod differs in its helical parameters from the structure determined previously by a hybrid approach. We provide evidence that these structural differences originate from different quaternary structures of pili assembled in vivo and in vitro.

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Date Issued

2017-12-05

Date Acceptance

2017-10-23

Citation

Structure, 2017, 25 (12), pp.1829-1839.e4

URI

http://hdl.handle.net/10044/1/103697

URL

https://www.sciencedirect.com/science/article/pii/S0969212617303325?via%3Dihub

DOI

https://www.dx.doi.org/10.1016/j.str.2017.10.004

ISSN

0969-2126

Publisher

Elsevier

Start Page

1829

End Page

1839.e4

Journal / Book Title

Structure

Volume

25

Issue

12

Copyright Statement

© 2017 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

License URL

http://creativecommons.org/licenses/by/4.0/

Identifier

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000417092100007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb

Subjects

ADHESIN

BACTERIAL OUTER-MEMBRANE

Biochemistry & Molecular Biology

BIOGENESIS

Biophysics

Cell Biology

COLI P-PILI

DICTIONARY

FIMBRIAE

FORCE

IDENTIFICATION

Life Sciences & Biomedicine

PATHWAY

PHYSICAL-PROPERTIES

Science & Technology

Publication Status

Published

Date Publish Online

2017-11-09