Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels
Author(s)
Type
Journal Article
Abstract
We explore the diagnostic potential of imaging endogenous fluorophores using two photon microscopy and fluorescence lifetime imaging (FLIM) in human skin with two spectral detection channels. Freshly excised benign dysplastic nevi (DN) and malignant nodular Basal Cell Carcinomas (nBCCs) were excited at 760 nm. The resulting fluorescence signal was binned manually on a cell by cell basis. This improved the reliability of fitting using a double exponential decay model and allowed the fluorescence signatures from different cell populations within the tissue to be identified and studied. We also performed a direct comparison between different diagnostic groups. A statistically significant difference between the median mean fluorescence lifetime of 2.79 ns versus 2.52 ns (blue channel, 300-500 nm) and 2.08 ns versus 1.33 ns (green channel, 500-640 nm) was found between nBCCs and DN respectively, using the Mann-Whitney U test (p < 0.01). Further differences in the distribution of fluorescence lifetime parameters and inter-patient variability are also discussed.
Date Issued
2011-12-01
Date Acceptance
2011-10-13
Citation
Biomedical Optics Express, 2011, 2 (12), pp.3295-3308
ISSN
2156-7085
Publisher
Optical Society of America
Start Page
3295
End Page
3308
Journal / Book Title
Biomedical Optics Express
Volume
2
Issue
12
Copyright Statement
©2011 Optical Society of America
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299883900007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Optics
Radiology, Nuclear Medicine & Medical Imaging
Biochemistry & Molecular Biology
IN-VIVO
MELANIN FLUORESCENCE
LASER TOMOGRAPHY
DIAGNOSIS
SPECTROSCOPY
MICROSCOPY
EXCITATION
CANCER
TISSUE
Publication Status
Published
Date Publish Online
2011-11-10