Synaptic secretion from human natural killer cells is diverse and includes supramolecular attack particles
Author(s)
Ambrose, Ashley R
Hazime, Khodor S
Worboys, Jonathan D
Niembro-Vivanco, Olatz
Davis, Daniel M
Type
Journal Article
Abstract
<jats:title>Significance</jats:title>
<jats:p>Cytotoxic immune cells kill cancer and virally infected cells through secretion of perforin and granzymes at immune synapses. It has recently been shown that cytotoxic T cells secrete membraneless protein structures, termed SMAPs, comprising perforin and granzyme within a shell of the glycoprotein TSP-1. Here, using a novel imaging technique termed "shadow imaging," we quantitatively assessed synaptic secretion from individual human NK cells. Upon ligation of activating receptors, NK cells secreted vesicles as well as membraneless SMAPs containing TSP-1, perforin, and granzyme B. Super-resolution microscopy revealed that NK cell SMAPs were larger than those secreted by T cells. Heterogeneity in NK cell synaptic secretion is likely important for the different effector functions of NK cell subtypes.</jats:p>
<jats:p>Cytotoxic immune cells kill cancer and virally infected cells through secretion of perforin and granzymes at immune synapses. It has recently been shown that cytotoxic T cells secrete membraneless protein structures, termed SMAPs, comprising perforin and granzyme within a shell of the glycoprotein TSP-1. Here, using a novel imaging technique termed "shadow imaging," we quantitatively assessed synaptic secretion from individual human NK cells. Upon ligation of activating receptors, NK cells secreted vesicles as well as membraneless SMAPs containing TSP-1, perforin, and granzyme B. Super-resolution microscopy revealed that NK cell SMAPs were larger than those secreted by T cells. Heterogeneity in NK cell synaptic secretion is likely important for the different effector functions of NK cell subtypes.</jats:p>
Date Issued
2020-09-22
Online Publication Date
2025-01-31T12:54:31Z
Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (38), pp.23717-23720
ISSN
0027-8424
Publisher
NATL ACAD SCIENCES
Start Page
23717
End Page
23720
Journal / Book Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume
117
Issue
38
License URI
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000575887200011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
PII: 2010274117
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
natural killer cells
immune synapse
supramolecular attack particles
SERGLYCIN
Publication Status
Published
Country
United States
Date Publish Online
2020-09-08