A measurement of the charge asymmetry in beauty-dijet production at LHCb is presented in this thesis. The measurement uses the 2016 dataset of proton-proton collisions gathered by the detector, corresponding to an integrated luminosity of 1.7 fb-1. The charge asymmetry is measured in three bins of the invariant dijet mass, with bin edges at: [50, 75, 105, 150] GeV. This represents the first measurement of the charge asymmetry in beauty-dijet production in proton-proton collisions at a centre-of-mass energy of 13 TeV. To make the measurement a charge tagging method is first developed and tested in order to distinguish between the presence of b- and anti-b-quarks in jets. The calibration of simulated events is then carried out to correct for mismodelling when compared to the data. Next, fits are performed to extract the yield of beauty-dijets in the data sample. These yields are then corrected for detector and mistagging effects. The values of the asymmetry are then calculated. Results are compared to Standard Model predictions and are found to agree to within one standard deviation in all three bins.