Comparing acute and chronic human cutaneous leishmaniasis caused by Leishmania major and Leishmania tropica focusing on arginase activity.
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
BACKGROUND: Cutaneous leishmaniasis (CL) in Iran is mainly caused by Leishmania major (L. major) and L. tropica. Arginase mediated L-arginine metabolism is an important issue in Leishmania parasite propagation. Arginase activity in human CL due to L. major and L. tropica have not been studied up to now. OBJECTIVES: We aimed to compare the clinical and laboratory aspects of acute and chronic CL, focussing on arginase activity. METHODS: In this case-control study, 30 patients with acute CL (duration ≤ 1 year), 13 patients with chronic CL (duration ≥ 2 year) and 11 healthy controls were recruited. Arginase activity was measured in skin biopsies of lesions, peripheral blood polymorphonuclear cells (PMNs), peripheral blood mononuclear cells (PBMCs) and plasma by standard methods. RESULTS: The median of arginase activity in the acute lesions was higher than in chronic samples and significantly higher than in healthy controls (P = 0.008). PMNs of both acute and chronic patients showed higher levels of arginase activity as compared to the levels in PBMCs and plasma. The median of arginase activity in the PMNs of patients with chronic CL was higher than that of patients with acute CL and significantly higher than that of the healthy controls (P = 0.010). CONCLUSION: The level of arginase activity in lesions of patients with acute and chronic CL was higher than the skin of healthy controls. The highest level of arginase activity was observed in PMNs from patients with chronic CL. This suggests that the high level of arginase activity in PMNs of patients with chronic CL may contribute to the chronicity.
Date Issued
2016-12-01
Date Acceptance
2016-05-19
Citation
Journal of the European Academy of Dermatology and Venereology, 2016, 30 (12), pp.2118-2121
ISSN
1468-3083
Publisher
Wiley
Start Page
2118
End Page
2121
Journal / Book Title
Journal of the European Academy of Dermatology and Venereology
Volume
30
Issue
12
Copyright Statement
© 2016 European Academy of Dermatology and Venereology. This is the pre-peer reviewed version of the following article, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1111/jdv.13838
Subjects
Science & Technology
Life Sciences & Biomedicine
Dermatology
INFECTION
MACROPHAGES
METABOLISM
GROWTH
Acute Disease
Arginase
Case-Control Studies
Chronic Disease
Humans
Leishmania major
Leishmania tropica
Leishmaniasis, Cutaneous
1103 Clinical Sciences
Dermatology & Venereal Diseases
Publication Status
Published
Date Publish Online
2016-07-21