Citrobacter rodentium relies on commensals for colonization of the colonic mucosa
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
We investigated the role of commensals at the peak of infection with the colonic mouse pathogen Citrobacter rodentium. Bioluminescent and kanamycin (Kan)-resistant C. rodentium persisted avirulently in the cecal lumen of mice continuously treated with Kan. A single Kan treatment was sufficient to displace C. rodentium from the colonic mucosa, a phenomenon not observed following treatment with vancomycin (Van) or metronidazole (Met). Kan, Van, and Met induce distinct dysbiosis, suggesting C. rodentium relies on specific commensals for colonic colonization. Expression of the master virulence regulator ler is induced in germ-free mice, yet C. rodentium is only seen in the cecal lumen. Moreover, in conventional mice, a single Kan treatment was sufficient to displace C. rodentium constitutively expressing Ler from the colonic mucosa. These results show that expression of virulence genes is not sufficient for colonization of the colonic mucosa and that commensals are essential for a physiological infection course.
Editor(s)
Frankel, GM
Date Issued
2017-12-19
Date Acceptance
2017-10-01
Citation
Cell Reports, 2017, 21 (12), pp.3381-3389
ISSN
2211-1247
Publisher
Elsevier
Start Page
3381
End Page
3389
Journal / Book Title
Cell Reports
Volume
21
Issue
12
Copyright Statement
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Subjects
AMR
C. rodentium
antibiotics
colonization resistance
enteric infection
microbiota
Publication Status
Published