Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Abscisic acid (ABA) is an important phytohormone with diverse applications. It currently relies on the fermentation of Botrytis cinerea, which suffers from limited availability of genetic engineering tools. Here, Yarrowia lipolytica was engineered to enable de novo biosynthesis of ABA. To overcome the rate-limiting P450 enzymes, systematic engineering strategies were implemented. Firstly, the dissolved oxygen was increased to boost the activity of P450 enzymes. Secondly, the expansion of endoplasmic reticulum was implemented to improve the functional expression of P450 enzymes. Lastly, rate-limiting enzymes were assembled to facilitate substrate trafficking. Moreover, ABA production was further improved by strengthening the mevalonate pathway. Finally, the engineered strain produced 1221.45 mg/L of ABA in a 5-L bioreactor. The study provides effective approaches for alleviating rate-limiting P450 enzymes to enhance ABA production and achieve competitive industrial-level ABA production in Y. lipolytica.
Date Issued
2025
Date Acceptance
2024-10-17
Citation
Synthetic and Systems Biotechnology, 2025, 10 (1), pp.165-173
ISSN
2405-805X
Publisher
Elsevier BV
Start Page
165
End Page
173
Journal / Book Title
Synthetic and Systems Biotechnology
Volume
10
Issue
1
Copyright Statement
© 2024 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC
BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Identifier
https://doi.org/10.1016/j.synbio.2024.10.004
Publication Status
Published
Date Publish Online
2024-10-18