A hybrid pathway for self-sustained luminescence
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Published version
Author(s)
Palkina, Kseniia A
Karataeva, Tatiana A
Perfilov, Maxim M
Fakhranurova, Liliia I
Markina, Nadezhda M
Type
Journal Article
Abstract
The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence
imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis – a step
catalysed by a large fungal polyketide synthase that requires a post-translational modification for activity.
Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically
diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more
compact, not dependent on availability of machinery for post-translational modifications, and confers
autonomous bioluminescence in yeast, mammalian and plant hosts. The compact size of plant hispidin
synthases enables additional modes of delivery of autoluminescence, such as delivery with viral vectors.
imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis – a step
catalysed by a large fungal polyketide synthase that requires a post-translational modification for activity.
Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically
diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more
compact, not dependent on availability of machinery for post-translational modifications, and confers
autonomous bioluminescence in yeast, mammalian and plant hosts. The compact size of plant hispidin
synthases enables additional modes of delivery of autoluminescence, such as delivery with viral vectors.
Date Issued
2024-03
Date Acceptance
2024-02-01
Citation
Science Advances, 2024, 10 (10)
ISSN
2375-2548
Publisher
American Association for the Advancement of Science
Journal / Book Title
Science Advances
Volume
10
Issue
10
Copyright Statement
Copyright © 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
https://creativecommons.org/licenses/by/4.0/
This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
https://creativecommons.org/licenses/by/4.0/
This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.science.org/doi/10.1126/sciadv.adk1992
Publication Status
Published
Article Number
eadk1992
Date Publish Online
2024-03-08