Remote polar regions offer unique opportunities and significant challenges for antimicrobial resistance research in a near-pristine environment. While core microbiology techniques continue to have an important role in supporting environmental research, the severe cold climate presents considerable challenges to laboratory research. We explore adaptations required for core bacteriology investigations in polar regions on an unsupported remote expedition c. 600 km north of the Arctic Circle utilizing the National Collection of Type Culture bacterial strains. Methods of culture, microscopy, biochemical and phenotypic testing, vortex, and centrifuge techniques are explored. Across −21.5 to −41.0°C, culture was satisfactorily enabled using a solar-powered USB incubator and an electricity-free water-bath option utilizing white gas for a variety of standard culture media. Microscopy and biochemical tests supported organism identification. Phenotypic testing for carbapenemase-producing genes using lateral flow devices showed good performance without modification (Carba-5, 20/20 carbapenemase-producing organism tests, 100% sensitivity; 100/100 negative targets, 100% specificity). The modified centrifuge was enabled with a 3D printed disk and Dremel drill and microbial DNA extraction (ZymoBIOMICS) kits were able to extract DNA of suitable quality for analysis. With suitable adaptations, conducting core microbiology techniques (with potential relevance for more advanced techniques) is possible in the remote extreme cold environment.