Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles
File(s)
Author(s)
Type
Journal Article
Abstract
pH homeostasis is strictly controlled at a subcellular level. A deregulation of the intra/extra/subcellular pH environment is associated with a number of diseases and as such, the monitoring of the pH state of cells and tissues is a valuable diagnostic tool. To date, only a few tools have been developed to measure the pH in living cells with the spatial resolution needed for intracellular imaging. Among the techniques available, only optical imaging offers enough resolution and biocompatibility to be proposed for subcellular pH monitoring. We present herein a ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells. This system provides the properties required for live cell quantitative imaging i.e. positive cellular uptake, biocompatibility, long wavelength excitation, sensitive response to pH within a biologically relevant range, and self-referenced signal.
Date Issued
2017-10-28
Date Acceptance
2017-09-25
Citation
Dalton Transactions, 2017, 46 (40), pp.13957-13965
ISSN
1477-9234
Publisher
Royal Society of Chemistry
Start Page
13957
End Page
13965
Journal / Book Title
Dalton Transactions
Volume
46
Issue
40
Copyright Statement
© The Royal Society of Chemistry 2017
Subjects
Science & Technology
Physical Sciences
Chemistry, Inorganic & Nuclear
Chemistry
UP-CONVERSION NANOPARTICLES
FLUORESCENT-PROBE
OPTICAL-PROPERTIES
CELLS
CANCER
NANOCRYSTALS
TRANSITION
CHALLENGES
EXCHANGER
EMISSION
Publication Status
Published