Nasosorption is a minimally invasive diagnostic procedure for measurement of viral load and markers of mucosal inflammation in RSV bronchiolitis
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Published version
Author(s)
Type
Journal Article
Abstract
Background.
Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.
Methods.
Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).
Results.
Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).
Conclusions.
Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.
Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.
Methods.
Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).
Results.
Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).
Conclusions.
Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.
Date Issued
2017-03-27
Date Acceptance
2017-03-20
Citation
The Journal of Infectious Diseases, 2017, 215 (8), pp.1240-1244
ISSN
1537-6613
Publisher
Oxford University Press (OUP)
Start Page
1240
End Page
1244
Journal / Book Title
The Journal of Infectious Diseases
Volume
215
Issue
8
Copyright Statement
© The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of
America. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted
reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
America. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted
reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
National Institute for Health Research
National Institute for Health Research
Commission of the European Communities
Grant Number
NF-SI-0513-10150
IS-HPU-1112-10064
116019
Subjects
Science & Technology
Life Sciences & Biomedicine
Immunology
Infectious Diseases
Microbiology
respiratory syncytial virus
nasosorption
viral load
bronchiolitis
respiratory sampling
RESPIRATORY SYNCYTIAL VIRUS
DISEASE SEVERITY
INFANTS
INFECTION
CHILDREN
respiratory sampling.
Bronchiolitis, Viral
Case-Control Studies
Chemokine CCL5
Female
Humans
Infant
Inflammation
Interferon-gamma
Interleukins
London
Male
Nasal Mucosa
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human
Viral Load
11 Medical And Health Sciences
06 Biological Sciences
Publication Status
Published