Genetic engineering of Ehrlich pathway modulate production of higher alcohols in engineered Yarrowia lipolytica
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Microbial cells can produce a vast spectrum of chemical compounds, including those most desired by the global chemical market, as for example higher alcohols, which are promising alternative fuels and chemical feedstock. In the current research, we investigated the effects of the Ehrlich pathway genetic engineering on higher alcohols production in Y. lipolytica, which directly follows our previous findings concerning elucidation of putative molecular identities involved in this pathway. To this end, we constructed two alternative expression cassettes composed of previously identified genes, putatively involved in the Ehrlich pathway in Y. lipolytica, and cloned them under the control of constitutive pTEF promoter, and by this-released them from extensive native regulation. The effects of the pathway engineering were investigated upon provision of different, Ehrlich pathway-inducing amino acids (L-Phe, L-Leu, L-Ile and L-Val). In general, amplification of the Ehrlich pathway in many cases led to increased formation of a respective higher alcohol from its precursor. We observed interesting effects of aminotransferase BAT2 deletion on synthesis of 2-phenylethanol and its acetate ester, significant relationship between L-Val and L-Phe catabolic pathways, and extensive 'cross-induction' of the derivative compounds synthesis by non-direct precursors.
Date Issued
2019-03-01
Date Acceptance
2018-11-01
Citation
FEMS Yeast Research, 2019, 19 (2)
ISSN
1567-1356
Publisher
Oxford University Press (OUP)
Journal / Book Title
FEMS Yeast Research
Volume
19
Issue
2
Copyright Statement
© 2018 Oxford University Press. This is a pre-copy-editing, author-produced version of an article accepted for publication in FEMS Yeast Research following peer review. The definitive publisher-authenticated version Ewelina Celińska, Monika Borkowska, Wojciech Białas, Monika Kubiak, Paulina Korpys, Marta Archacka, Rodrigo Ledesma-Amaro, Jean-Marc Nicaud; Genetic engineering of Ehrlich pathway modulate production of higher alcohols in engineered Yarrowia lipolytica, FEMS Yeast Research, , foy122, is available online at: https://dx.doi.org/10.1093/femsyr/foy122
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/30452758
PII: 5188678
Subjects
06 Biological Sciences
10 Technology
09 Engineering
Microbiology
Publication Status
Published
Coverage Spatial
England
Article Number
foy122
Date Publish Online
2018-11-18