Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers
File(s)
Accepted version
Author(s)
Roberts, Rosalind F
Bengoa-Vergniory, Nora
Alegre-Abarrategui, Javier
Type
Journal Article
Abstract
Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson's disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.
Date Issued
2019-02-16
Date Acceptance
2019-02-01
Citation
Methods in Molecular Biology, 2019, 1948, pp.69-76
ISSN
1940-6029
Publisher
Humana Press
Start Page
69
End Page
76
Journal / Book Title
Methods in Molecular Biology
Volume
1948
Copyright Statement
© 2019 Springer Science+Business Media, LLC, part of Springer Nature. The final publication is available at Springer via https://dx.doi.org/10.1007/978-1-4939-9124-2_7
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/30771171
Subjects
Aggregates
Alpha-synuclein
Oligomers
Parkinson’s disease
Pathology
Proximity ligation assay
Synucleinopathy
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2019-02-16