easySLM-STED: stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning
File(s)G-rlitz_et_al-2018-Journal_of_Biophotonics.pdf (3.98 MB)
Published version
Author(s)
Type
Journal Article
Abstract
We demonstrate a simplified set‐up for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to extend the field of view for STED microscopy by correcting chromatic aberration that otherwise leads to walk‐off between the focused excitation and depletion beams. We further show how this arrangement can be adapted to increase the imaging speed through multibeam excitation and depletion. Fine adjustments to the alignment can be accomplished using the SLM only, conferring the potential for automation.
Date Issued
2018-11-01
Date Acceptance
2018-07-05
Citation
Journal of Biophotonics, 2018, 11 (11)
ISSN
1864-063X
Publisher
Wiley-VCH Verlag
Journal / Book Title
Journal of Biophotonics
Volume
11
Issue
11
Copyright Statement
© 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
Sponsor
Medical Research Council
Medical Research Council (MRC)
Grant Number
MR/K015834/1
MR/K015834/1
Subjects
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Biophysics
Optics
Biochemistry & Molecular Biology
aberration correction
spatial light modulator
STED
super resolved microscopy
FLUORESCENCE MICROSCOPY
RESOLUTION
BREAKING
BARRIER
DESIGN
STED
aberration correction
spatial light modulator
super resolved microscopy
Optoelectronics & Photonics
Publication Status
Published
Article Number
e201800087
Date Publish Online
2018-07-05