Wettability design is of crucial importance for the optimization of multiphase flow behaviour in gas diffusion layers (GDLs) in fuel cells. The accumulation of electrochemically-generated water in the GDLs will impact fuel cell performance. Hence, it is necessary to understand multiphase displacement to design optimal pore structures and wettability to allow the rapid flow of gases and water in GDLs over a wide saturation range. This work uses high-resolution in situ three-dimensional X-ray imaging combined with a pore network model to investigate the breakthrough capillary pressure and water saturation in GDLs manufactured with different mass fractions of polytetrafluoroethylene coating: 5, 20, 40, and 60%, making them more hydrophobic. We first demonstrate that the pore network extraction method provides representative networks for the fibrous porous media examined. Then, using a pore-network flow model we simulate water invasion into initially gas-filled fibrous media, and analyze the effect of wettability on breakthrough capillary pressure and water saturation. With an appropriate pore-scale characterization of wettability, a pore network model can match experimental results and predict displacement behaviour.