Assessing corneal biomechanics with Brillouin spectro-microscopy
File(s)
Accepted version
Author(s)
Lepert, G
Gouveia, RM
Connon, CJ
Paterson, C
Type
Journal Article
Abstract
A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.
Date Issued
2016-06-01
Date Acceptance
2015-12-07
Citation
Faraday Discussions, 2016, 187 (7), pp.415-428
ISSN
1364-5498
Publisher
Royal Society of Chemistry
Start Page
415
End Page
428
Journal / Book Title
Faraday Discussions
Volume
187
Issue
7
Copyright Statement
© The Royal Society of Chemistry 2016
Sponsor
Medical Research Council (MRC)
Grant Number
MR/K017217/1 Reading:H5167900
Subjects
Chemical Physics
0306 Physical Chemistry (Incl. Structural)
0904 Chemical Engineering
Publication Status
Published
Date Publish Online
2016-04-06