Dynamical model fitting to a synthetic positive feedback circuit in E. coli
File(s)enb.2020.0009.pdf (1.59 MB)
Published version
Author(s)
Tica, Jure
Zhu, Tong
Isalan, Mark
Type
Journal Article
Abstract
Applying the principles of engineering to Synthetic Biology relies on the development of robust and modular genetic components, as well as underlying quantitative dynamical models that closely predict their behaviour. This study looks at a simple positive feedback circuit built by placing filamentous phage secretin pIV under a phage shock promoter. A single-equation ordinary differential equation model is developed to closely replicate the behaviour of the circuit, and its response to inhibition by TetR. A stepwise approach is employed to fit the model's parameters to time-series data for the circuit. This approach allows the dissection of the role of different parameters and leads to the identification of dependencies and redundancies between parameters. The developed genetic circuit and associated model may be used as a building block for larger circuits with more complex dynamics, which require tight quantitative control or tuning.
Date Issued
2020-06-01
Date Acceptance
2020-05-22
Citation
Engineering Biology, 2020, 4 (2), pp.25-31
ISSN
2398-6182
Publisher
Institution of Engineering and Technology (IET)
Start Page
25
End Page
31
Journal / Book Title
Engineering Biology
Volume
4
Issue
2
Copyright Statement
© 2020 The Institution of Engineering and Technology
This is an open access article published by the IET under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/)
This is an open access article published by the IET under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/)
License URL
Sponsor
Wellcome Trust
Wellcome Trust
Biotechnology and Biological Sciences Research Council (BBSRC)
VolkwagenStiftung
Grant Number
102944/Z/13/Z
102944/Z/13/Z
BB/P020615/1
63062
Publication Status
Published
Date Publish Online
2020-06-23