RAPD PCR detects co-colonisation of multiple Group B Streptococcus genotypes: a practical molecular technique for screening multiple colonies
File(s)
Published version
Supporting information
Author(s)
Type
Journal Article
Abstract
Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.
Date Issued
2021-11-01
Date Acceptance
2021-09-03
Citation
Journal of Microbiological Methods, 2021, 190
ISSN
0167-7012
Publisher
Elsevier
Journal / Book Title
Journal of Microbiological Methods
Volume
190
Copyright Statement
Crown Copyright © 2021 Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
License URL
Sponsor
Rosetrees Trust
Subjects
Co-colonisation
Genetic diversity
Group B Streptococcus
Multiple serotypes
RAPD PCR
S. agalactiae
Screening
0605 Microbiology
1108 Medical Microbiology
Microbiology
Publication Status
Published
Article Number
ARTN 106322
Date Publish Online
2021-09-08